The Impact of Latino Family Variables on the Sexual Activity of Latino Adolescents: A Mixed-Methods Study

Researchers\u27 examination of Latino adolescents\u27 cultural values and sexual activity has yielded questions regarding the cultural- and gender-specific attitudes and practices in this area (Deardorff, Tschann, & Flores, 2008). Cultural values include family-related variables such as different aspects of familism, parent-adolescent communication, and parental monitoring, which have been found to decrease adolescents\u27 engagement in other negative activities such as aggressive behavior, (Dishion & McMahon, 1998), substance use (Estrada, Rabow, & Watts, 1982), and juvenile delinquency (Clark & Shields, 1997). Research investigating these risk behaviors has often implicated Latino adolescents\u27 level of assimilation to White, mainstream society as a potential risk factor for higher engagement in negative behaviors, emphasizing the importance of better understanding the cultural context in which Latino youth live and how it may impact their risky behavior. This dissertation study, therefore, examined the relationship between five Latino family variables (i.e., attitudinal familism, behavioral familism, structural familism, parent-adolescent communication, and parental monitoring), assimilation to White culture, and the sexual activity beliefs and behaviors of Latino adolescents. A mixed-methods project explored and tested the relationships between these variables. Four gender-specific focus groups were conducted with adolescents; data were analyzed using Grounded Theory methodology (Strauss & Corbin, 1990). Also, 410 Latino youth completed quantitative surveys about their family-related behaviors, attitudes, and sexual activity. Relationships among these variables were tested using correlations and regressions. Primary findings from the qualitative study indicated that gender differences in Latino families\u27 communication about sex and monitoring behaviors impacts Latina girls\u27 and Latino boys\u27 sexual activity beliefs. Differences observed with regard to gender were such that Latina girls\u27 felt sexual activity was not appropriate for teenagers, whereas the boys felt that it was acceptable for teenagers to engage in sexual activity as long as they used a contraceptive method. Quantitative results indicated parental monitoring and some aspects of familism were found to be related to a decreased likelihood of engaging in sexual activity for the girls and boys. High assimilation to White, mainstream culture was not found to impact sexual activity beliefs or behaviors in the current study

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Effect of angiotensin-converting enzyme inhibitor and angiotensin receptor blocker initiation on organ support-free days in patients hospitalized with COVID-19

IMPORTANCE Overactivation of the renin-angiotensin system (RAS) may contribute to poor clinical outcomes in patients with COVID-19. Objective To determine whether angiotensin-converting enzyme (ACE) inhibitor or angiotensin receptor blocker (ARB) initiation improves outcomes in patients hospitalized for COVID-19. DESIGN, SETTING, AND PARTICIPANTS In an ongoing, adaptive platform randomized clinical trial, 721 critically ill and 58 non–critically ill hospitalized adults were randomized to receive an RAS inhibitor or control between March 16, 2021, and February 25, 2022, at 69 sites in 7 countries (final follow-up on June 1, 2022). INTERVENTIONS Patients were randomized to receive open-label initiation of an ACE inhibitor (n = 257), ARB (n = 248), ARB in combination with DMX-200 (a chemokine receptor-2 inhibitor; n = 10), or no RAS inhibitor (control; n = 264) for up to 10 days. MAIN OUTCOMES AND MEASURES The primary outcome was organ support–free days, a composite of hospital survival and days alive without cardiovascular or respiratory organ support through 21 days. The primary analysis was a bayesian cumulative logistic model. Odds ratios (ORs) greater than 1 represent improved outcomes. RESULTS On February 25, 2022, enrollment was discontinued due to safety concerns. Among 679 critically ill patients with available primary outcome data, the median age was 56 years and 239 participants (35.2%) were women. Median (IQR) organ support–free days among critically ill patients was 10 (–1 to 16) in the ACE inhibitor group (n = 231), 8 (–1 to 17) in the ARB group (n = 217), and 12 (0 to 17) in the control group (n = 231) (median adjusted odds ratios of 0.77 [95% bayesian credible interval, 0.58-1.06] for improvement for ACE inhibitor and 0.76 [95% credible interval, 0.56-1.05] for ARB compared with control). The posterior probabilities that ACE inhibitors and ARBs worsened organ support–free days compared with control were 94.9% and 95.4%, respectively. Hospital survival occurred in 166 of 231 critically ill participants (71.9%) in the ACE inhibitor group, 152 of 217 (70.0%) in the ARB group, and 182 of 231 (78.8%) in the control group (posterior probabilities that ACE inhibitor and ARB worsened hospital survival compared with control were 95.3% and 98.1%, respectively). CONCLUSIONS AND RELEVANCE In this trial, among critically ill adults with COVID-19, initiation of an ACE inhibitor or ARB did not improve, and likely worsened, clinical outcomes. TRIAL REGISTRATION ClinicalTrials.gov Identifier: NCT0273570

Latino Family Variables and Sexual Activity in Latino Adolescents

Researchers’ focused examination of Latino adolescents’ cultural values and sexual activity has yielded questions regarding the cultural- and gender-specific attitudes and practices of these youth (Deardorff, Tschann, & Flores, 2008). Cultural values include family-related variables such as different aspects of familism, parent-adolescent communication, and parental monitoring, which have been found to decrease adolescents’ engagement in other negative activities such as aggressive behavior, (Dishion & McMahon, 1998), substance use (Estrada, Rabow, & Watts, 1982), and delinquency (Clark & Shields, 1997). Research investigating these risk behaviors has often implicated Latino adolescents’ level of assimilation to White, mainstream society as a potential risk factor for higher engagement in negative behaviors. This dissertation study, therefore, will examine the relationship between five Latino family variables (i.e., attitudinal familism, behavioral familism, structural familism, parent-adolescent communication, and parental monitoring), assimilation to White society, and the sexual activity of Latino adolescents. A mixed-methods project has been designed to explore and test the relationships between these variables. Focus groups will be conducted with adolescents on the topics of family, gender, and sexual activity; data will be analyzed using Grounded Theory methodology (Strauss & Corbin, 1990). Latino youth will also complete surveys about these topics; the relationships among these variables will be explored statistically. For the purposes of the Research Exchange, a description of the current stage of the project at that time, a discussion of the anticipated results of the study, and an explanation of the contributions of this study to the Milwaukee and psychological communities will be included